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Last updated 2/12
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Perkin Elmer AAnalyst 200 Atomic Absorption Spectrophotometer
What it does:
Atomic absorption spectrophotometry provides accurate quantitative
analyses for metals in water, sediments, soils or rocks. (Samples are
analyzed in solution form, so solid samples must be leached or dissolved prior to analysis.)
How it works:
Atomic absorption units have four basic parts: interchangeable lamps that
emit light with element-specific wavelengths, a sample aspirator, a flame or furnace apparatus for
volatilizing the sample, and a photon detector. In order to analyze for any given element, a lamp
is chosen that produces a wavelength of light that is absorbed by that element. Sample solutions
are aspirated into the flame. If any ions of the given element are present in the flame, they will
absorb light produced by the lamp before it reaches the detector. The amount of light absorbed depends on
the amount of the element present in the sample. Absorbance values for unknown samples are compared to
calibration curves prepared by running known samples.
Instrument Statistics:
Our system combines a double-beam Echelle optical system with a
solid state detector. Both acetylene and nitrous oxide fuel mixtures can be used to allow for the analysis
of a wide range of elements. Cordless LuminaTM lamps provide the element specific
light. The system is run by WinLab 32TM operating software with touch-screen controls.
Related Research:
This lab has been used extensively to collect data for a wide variety
of research. Some of the major projects include:
- CZEN - Study on Soil Development & Chemical Weathering (Project Website)
- NSF/CRUI - Study on Acid Deposition and Calcium Depletion in Adirondack Soils
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