Controlled Rotation & Translation of Microorganisms in an Optical Trap

Adam Weiss

Advisor: Dr. Enrique Galvez

Using a single beam gradient trap, we can trap, translate, and rotate yeast cells. A laser that produces high-order transverse modes is used in a laser tweezers setup to control the rotational orientation of irregular objects, including microorganisms. Our system allows for full control of objects in two dimensions. We describe our system, including results for transverse trapping power and torque, as well as calculations from a model system. This technique could be very useful to biologists in the study of microscopic life.